2011.06.24 20:40 "[Tiff] 16-bit image", by Leandro Bugnon

2011.06.25 00:27 "Re: [Tiff] 16-bit image", by Leandro Bugnon

The image was made that way intentionally. My problem is that if I have a stack with 2 images or slices, some of the viewers I've tryed define the display range between the min and max of the first image. In the test image,

 the value 500 is represented in black and 700 in white, so the second
image (1000-1400) it's completely white.

I cannot scale the image becouse it's for cuantitative microscopy, and the values goes from 0 to 2^14, so if I have a image from 500-700, it have to be almost dark and darker than the second image. I supposed that had some tag missed, but it seems not. The pixel values are correctly saved, the problems seems the visualization info to show them in a scale from 0 to 2^16 ( I can scale from 0-2^14 to 0-2^16 but I cannot scale anything to 0-2^16 becouse I'm distorting the information).

Thanks for your answers and sorry for my english

2011/6/24 Chris Cox <ccox@adobe.com>

The min/max sample values are metadata, and don’t affect interpretation of the file data.

So even if wrong, they shouldn’t cause the symptoms described.

I do wonder if the byte order of the data might be wrong, and it’s picking up the low order bits of the image.

On 6/24/11 3:50 PM, "Leandro Bugnon" <l.bugnon@gmail.com> wrote:

Thanks for the quick answer. Here is a test image

-   http://www.filefactory.com/file/cc0b359/n/test1.tif

Another thing that cannot understand is that TIFFTAG_SMINSAMPLEVALUE / TIFFTAG_SMAXSAMPLEVALUE remains in zero value but I changed that in the code.

2011/6/24 Bob Friesenhahn <bfriesen@simple.dallas.tx.us>

Hi,

I'm getting a 14bit image from a CCD cam, and save it in a 16bit. The
problem is that, if I take only 2 gray

values (greater than zero and less
than 2^14), the viewers that I try (imageJ and irfanview) take them as

zero
and max valuo (black and white), they don't show the real color. How can I
fix it? I've tryed the next

tags:

The values are correctly saved, but it always show a full black and full
white value when there isn't any

zero or 2^16 value.

I am not immediately seeing a problem with your tags. Perhaps the pixel values are wrong, or perhaps this is a problem with these viewers.

Are you able to post a sample TIFF file somewhere so we can download it and see how it is constructed?

--

Leandro